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Related Concept Videos

Phosphorylation01:02

Phosphorylation

55.7K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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Phosphorylation01:02

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Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
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Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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Protein Kinases and Phosphatases02:54

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The Phragmoplast01:59

The Phragmoplast

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Cell division is essential for organismal growth and development. In animal cells, the central spindle and its associated proteins form the midbody, a structure that has an essential role in cytokinesis. In plants, the central spindle, along with the microtubules, actin, and other cell components, matures into the phragmoplast, which is necessary for cytokinesis. Unlike the stationary midbody, the phragmoplast expands centrifugally, eventually leading to the formation of the new cell wall.
The...
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Updated: Apr 13, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
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Phosphorylation site prediction in plants.

Qiuming Yao1, Waltraud X Schulze, Dong Xu

  • 1Department of Computer Science and Bond Life Sciences Center, University of Missouri, Columbia, MO, 65211, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 2, 2015
PubMed
Summary
This summary is machine-generated.

Computational tools accelerate plant phosphoproteome analysis. These in silico methods predict protein phosphorylation sites, saving time and cost compared to experimental screening, aiding plant molecular biology research.

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Identification of Post-translational Modifications of Plant Protein Complexes
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Area of Science:

  • Plant molecular biology
  • Proteomics
  • Bioinformatics

Background:

  • Protein phosphorylation is crucial for plant signaling, modifying serine, threonine, and tyrosine residues.
  • Experimental proteome-wide phosphorylation screening is time-consuming and expensive.
  • In silico prediction methods offer a complementary approach to experimental studies.

Purpose of the Study:

  • To introduce and evaluate plant-specific in silico phosphorylation site prediction tools.
  • To provide technical details and application guidelines for these computational tools.
  • To assess the performance of Musite, PhosPhAt, and PlantPhos using Arabidopsis data.

Main Methods:

  • Focus on in silico prediction tools for plant phosphorylation sites.
  • Utilize statistical models trained on experimental phosphorylation data.
  • Apply and compare three representative tools: Musite, PhosPhAt, and PlantPhos.

Main Results:

  • Demonstrated the practical application of computational methods for large-scale phosphorylation identification.
  • Presented prediction results for Arabidopsis protein phosphorylation events.
  • Highlighted the strengths and limitations of the evaluated prediction tools.

Conclusions:

  • In silico prediction tools are fast, cost-effective, and practical for plant phosphoproteome analysis.
  • These tools can enhance phosphorylation site identification, hypothesis generation, and experimental design.
  • The evaluated tools are valuable resources for the plant phosphorylation research community.