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Updated: Apr 13, 2026
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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
Published on: October 1, 2014
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Three dimensional time-gated tracking of non-blinking quantum dots in live cells
Matthew S DeVore1, Dominik G Stich1, Aaron M Keller1
1Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Summary
Researchers developed a new 3D tracking microscope with non-blinking quantum dots to overcome limitations in observing single particle tracking in live cells. This allows for longer observation times and improved signal-to-noise ratio in cellular environments.
Area of Science:
- Biophysics
- Cell Biology
- Nanotechnology
Background:
- Single particle tracking is crucial for studying cellular processes.
- Limitations include probe blinking and out-of-plane motion, restricting observation duration.
Purpose of the Study:
- To overcome limitations in single particle tracking.
- To enable longer observation times of cellular dynamics in 3D.
Main Methods:
- Utilized novel non-blinking quantum dots as fluorescent probes.
- Employed a custom-built 3D tracking microscope for active particle following.
- Implemented pulsed excitation and time-gated detection to enhance signal-to-noise ratio.
Main Results:
- Successfully tracked single particles in live cells for extended periods.
- Overcame limitations of probe blinking and out-of-plane motion.
- Achieved improved signal-to-noise ratio in complex cellular environments.
Conclusions:
- The developed system significantly advances the capabilities of single particle tracking.
- Enables deeper insights into biophysical processes within live cells.
- Offers a robust method for studying motor protein transport and membrane diffusion.
