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Updated: Jan 1, 2026

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens

Published on: June 6, 2020

Rapid, optimized interactomic screening.

Zhanna Hakhverdyan¹, Michal Domanski², Loren E Hough¹

¹Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, New York, USA.

|May 5, 2015

Summary

This summary is machine-generated.

This study introduces a novel screening method to discover how to capture macromolecular complexes and their binding partners. This approach systematically dissects cellular interactomes for a deeper understanding of biological systems.

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Area of Science:

Biochemistry
Molecular Biology
Systems Biology

Background:

Mapping cellular macromolecular complexes and their interactions is crucial for understanding biological systems.
Current methods lack the ability to accurately predict how to capture specific macromolecular complexes with their physiological binding partners.

Purpose of the Study:

To develop and present a screening method for comprehensively exploring parameters that affect the stability of interactions in affinity-captured complexes.
To enable the discovery of physiological binding partners in unprecedented detail.

Main Methods:

Developed a screening method to systematically explore parameters influencing the stability of interactions within affinity-captured complexes.
Applied this screening approach to various macromolecular complexes across different organisms.

Main Results:

The screening method comprehensively explores interaction parameters, leading to the discovery of physiological binding partners.
Novel interaction profiles were revealed for both well-studied and less-characterized proteins.
The approach demonstrated robustness, cost-effectiveness, and automation potential.

Conclusions:

The presented method offers a robust, economical, and automatable solution for dissecting cellular interactomes.
This systematic approach provides new avenues for detailed analysis of protein-protein interactions and complex formation.