Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Queuine is incorporated into brain transfer RNA.

T J Siard1, J R Katze, W R Farkas

  • 1University of Tennessee College of Veterinary Medicine, Knoxville 37901-1071.

Neurochemical Research
|November 1, 1989
PubMed
Summary

Queuine, a modified guanine base, is present in nearly all Q-family tRNAs in pig brains. This indicates queuine crosses the blood-brain barrier and the brain has the necessary enzyme for this base modification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit.

Gene·2001
Same author

The role of environmental antigens in the spontaneous development of autoimmunity in MRL-lpr mice.

Journal of immunology (Baltimore, Md. : 1950)·1999
Same author

Biosynthesis of archaeosine, a novel derivative of 7-deazaguanosine specific to archaeal tRNA, proceeds via a pathway involving base replacement on the tRNA polynucleotide chain.

The Journal of biological chemistry·1997
Same author

Effects of a diet deficient in tyrosine and queuine on germfree mice.

Biochemical and biophysical research communications·1997
Same author

Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit.

Archives of biochemistry and biophysics·1996
Same author

Absence of plasma amine oxidase in some frequently used animal models.

Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology·1993

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Neuroscience

Background:

  • Transfer RNA (tRNA) plays a crucial role in protein synthesis.
  • Modified nucleosides in tRNA, like queuosine, can impact translation fidelity.
  • The presence and role of queuosine in mammalian brain tRNA are not fully understood.

Purpose of the Study:

  • To investigate the presence and extent of queuosine modification in pig brain tRNA.
  • To determine if queuosine can cross the blood-brain barrier.
  • To identify the presence of guanine-queuine tRNA transglycosylase in the brain.

Main Methods:

  • Assaying pig brain tRNA for queuosine in the anticodon of Q-family tRNAs (Asp, Asn, His, Tyr).
  • Aminoacylating tRNA with the respective amino acids.
  • Analyzing aminoacylated tRNAs using RPC-5 chromatography.
  • Confirming queuosine presence via chemical modification (BrCN or NaIO4) and altered RPC-5 elution profiles.

Main Results:

  • Queuine was found to substitute for guanine in virtually 100% of the anticodons for all four Q-family tRNAs.
  • The elution profiles from RPC-5 chromatography confirmed the presence of queuine.
  • Chemical treatments (BrCN, NaIO4) resulted in predictable changes to the elution profiles, validating the findings.

Conclusions:

  • Queuine successfully crosses the blood-brain barrier into the pig brain.
  • The pig brain possesses active guanine-queuine tRNA transglycosylase, the enzyme responsible for guanine replacement with queuine.
  • This extensive modification suggests a significant role for queuosine in brain function and translation.

Related Experiment Videos