Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

STAT1-S727 - the license to kill.

Eva M Putz1, Dagmar Gotthardt1, Veronika Sexl1

  • 1Institute of Pharmacology and Toxicology; University of Veterinary Medicine Vienna ; Vienna, Austria.

Oncoimmunology
|May 6, 2015
PubMed
Summary

Cyclin-dependent kinase 8 (CDK8) phosphorylates signal transducer and activator of transcription 1 (STAT1), inhibiting natural killer cell activity. This suggests CDK8 is a potential target for cancer immunotherapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CDK6 inactivation counteracts CALR-mutant-induced MPN evolution and sensitizes MPN stem cells to interferon-α treatment.

HemaSphere·2026
Same author

CNOT3 supports ILC2 differentiation and function by destabilizing Tbx21 and Rorc transcripts.

The Journal of experimental medicine·2026
Same author

Innate lymphoid cells integrate sensing and plasticity to control fungal infections.

Cell reports·2026
Same author

Preclinical models of hepatosplenic γδ T-cell lymphoma with an activating STAT5B mutation display sensitivity to JAK inhibitor upadacitinib.

HemaSphere·2026
Same author

Editorial Expression of Concern: Increased NK cell immunity in a transgenic mouse model of NKp46 overexpression.

Scientific reports·2026
Same author

Extramedullary hematopoiesis in the spleen contributes to natural killer cell development during infection and inflammation.

Haematologica·2026

Area of Science:

  • Immunology
  • Molecular Biology
  • Cancer Research

Background:

  • Serine phosphorylation is crucial for STAT protein transcriptional activity.
  • Signal transducer and activator of transcription 1 (STAT1) plays a role in immune responses.
  • Natural killer (NK) cells are critical for tumor surveillance.

Purpose of the Study:

  • To investigate the role of CDK8-mediated phosphorylation of STAT1 on S727.
  • To determine the impact of this phosphorylation on NK cell cytotoxicity and tumor surveillance.
  • To evaluate CDK8 as a potential therapeutic target in immunotherapy.

Main Methods:

  • Analysis of STAT1 phosphorylation at S727.
  • Assessment of NK cell cytotoxicity assays.
  • Evaluation of tumor surveillance in relevant models.
Keywords:
CDK, cyclin-dependent kinaseCDK8CTLA-4, cytotoxic T lymphocyte antigen 4FDA, food and drug administrationKLRG1, killer cell lectin-like receptor subfamily G member 1MHC, major histocompatibility complexNK cellsNK, natural killerPD-1, programmed cell death 1STAT1STAT1, signal transducer and activator of transcription 1immunotherapytumor immune surveillance

Related Experiment Videos

Main Results:

  • CDK8-mediated phosphorylation of STAT1 on S727 was identified.
  • This specific phosphorylation was found to inhibit NK cell cytotoxicity.
  • The phosphorylation was also shown to restrain tumor surveillance, indicating a suppressive role.

Conclusions:

  • CDK8-mediated STAT1 phosphorylation at S727 acts as a negative regulator of anti-tumor immunity.
  • Targeting CDK8 may enhance NK cell-mediated tumor surveillance and improve immunotherapy outcomes.