Consensus guidelines for the detection of immunogenic cell death
Oliver Kepp1, Laura Senovilla2, Ilio Vitale3
1Equipe 11 labellisée par la Ligue Nationale contre le Cancer; Center de Recherche des Cordeliers ; Paris, France ; INSERM; U1138 ; Paris, France ; Metabolomics and Cell Biology Platforms; Gustave Roussy Cancer Campus ; Villejuif, France.
Oncoimmunology
|May 6, 2015
Summary
Immunogenic cell death (ICD) triggers immune responses, unlike typical apoptosis. New high-throughput methods detect ICD markers, aiding development of novel cancer therapies that turn tumor cells into vaccines.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Apoptotic cells are generally considered immune-tolerant.
- Certain stimuli induce a form of cell death known as immunogenic cell death (ICD).
- ICD involves the release of immunostimulatory damage-associated molecular patterns (DAMPs).
Conclusions:
- ICD has significant clinical and therapeutic implications in cancer treatment.
- The developed platform can accelerate the discovery of ICD-inducing anticancer agents.
- This technology may lead to cancer regimens that function as cancer-specific vaccines.
Keywords:
APC, antigen-presenting cellATF6, activating transcription factor 6ATP releaseBAK1, BCL2-antagonist/killer 1BAX, BCL2-associated X proteinBCL2, B-cell CLL/lymphoma 2 proteinCALR, calreticulinCTL, cytotoxic T lymphocyteDAMP, damage-associated molecular patternDAPI, 4′,6-diamidino-2-phenylindoleDiOC6(3), 3,3′-dihexyloxacarbocyanine iodideEIF2A, eukaryotic translation initiation factor 2AER, endoplasmic reticulumFLT3LG, fms-related tyrosine kinase 3 ligandG3BP1, GTPase activating protein (SH3 domain) binding protein 1GFP, green fluorescent proteinH2B, histone 2BHMGB1HMGB1, high mobility group box 1HSP, heat shock proteinHSV-1, herpes simplex virus type IICD, immunogenic cell deathIFN, interferonIL, interleukinMOMP, mitochondrial outer membrane permeabilizationPDIA3, protein disulfide isomerase family API, propidium iodideRFP, red fluorescent proteinTLR, Toll-like receptorXBP1, X-box binding protein 1autophagycalreticulinendoplasmic reticulum stressimmunotherapymember 3Δψm, mitochondrial transmembrane potential
