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Related Concept Videos

Nuclear Export01:42

Nuclear Export

5.3K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
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Nuclear Export of mRNA02:31

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Nuclear Fission02:50

Nuclear Fission

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Many heavier elements with smaller binding energies per nucleon can decompose into more stable elements that have intermediate mass numbers and larger binding energies per nucleon—that is, mass numbers and binding energies per nucleon that are closer to the “peak” of the binding energy graph near 56. Sometimes neutrons are also produced. This decomposition of a large nucleus into smaller pieces is called fission. The breaking is rather random with the formation of a large...
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Nuclear Transmutation03:20

Nuclear Transmutation

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Nuclear transmutation is the conversion of one nuclide into another. It can occur by the radioactive decay of a nucleus, or the reaction of a nucleus with another particle. The first manmade nucleus was produced in Ernest Rutherford’s laboratory in 1919 by a transmutation reaction, the bombardment of one type of nuclei with other nuclei or with neutrons. Rutherford bombarded nitrogen-14 atoms with high-speed α particles from a natural radioactive isotope of radium and observed...
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Related Experiment Video

Updated: Apr 13, 2026

Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence
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Detection of Nuclear Blebbing and DNA Leakage in Mammalian Cells by Immunofluorescence

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ESCRTs breach the nuclear border.

Brant M Webster1, C Patrick Lusk

  • 1a Yale School of Medicine ; New Haven , CT , USA.

Nucleus (Austin, Tex.)
|May 6, 2015
PubMed
Summary
This summary is machine-generated.

The endosomal sorting complexes required for transport (ESCRT)-III machinery plays a novel role in nuclear pore complex (NPC) biogenesis at the nuclear envelope (NE). This study reveals ESCRT-III

Keywords:
ERAD, ER-Associated DegradationESCRT, Endosomal Sorting Complexes Required for TransportINM, Inner Nuclear MembraneLEM, Lap2, Emerin, MAN1MVB, Multivesicular BodyNE, Nuclear EnvelopeNLS, Nuclear Localization SignalNPC, Nuclear Pore ComplexONM, Outer Nuclear MembraneSINC, Storage of Improperly assembled Nuclear pore Complexesendosomal sorting complex required for transportmembrane curvaturenuclear envelopenuclear pore complexquality control

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Single-Molecule Imaging of Nuclear Transport
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • The endosomal sorting complexes required for transport (ESCRT) machinery is crucial for membrane trafficking, particularly in sorting ubiquitylated proteins.
  • ESCRT-III, the most ancient ESCRT component, forms helical filaments that mediate membrane deformation and scission.
  • ESCRT-III functions in various cellular processes beyond endosomal sorting.

Purpose of the Study:

  • To investigate the role of ESCRT-III in nuclear pore complex (NPC) biogenesis.
  • To elucidate the mechanism of ESCRT-III involvement in nuclear envelope (NE) quality control.
  • To integrate ESCRT-III into emerging models of NPC formation and NE integrity.

Main Methods:

  • Utilized advanced microscopy techniques to visualize ESCRT-III at the NE.
  • Employed biochemical assays to study ESCRT-III interactions with NPC components.
  • Investigated the functional consequences of ESCRT-III depletion or mutation on NPC formation and NE structure.

Main Results:

  • Identified a novel role for ESCRT-III in a quality control pathway at the nuclear envelope.
  • Demonstrated that ESCRT-III is essential for the proper biogenesis of nuclear pore complexes.
  • Provided evidence for ESCRT-III's involvement in maintaining the integrity of the nuclear envelope.

Conclusions:

  • ESCRT-III machinery is implicated in nuclear pore complex assembly and quality control at the nuclear envelope.
  • This finding expands the known functions of ESCRT-III to nuclear biology.
  • ESCRT-III likely plays a broader role in ensuring nuclear compartment integrity.