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Related Experiment Video

Updated: Apr 13, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

Published on: April 22, 2014

Enhanced single-cell printing by acoustophoretic cell focusing.

I Leibacher¹, J Schoendube, J Dual¹

¹Institute of Mechanical Systems, Department of Mechanical and Process Engineering, Swiss Federal Institute of Technology (ETH) , Tannenstr. 3, CH-8092 Zurich, Switzerland.

Biomicrofluidics

|May 7, 2015

Summary

This study presents an improved single-cell printer using acoustophoretic focusing for precise cell handling. The new system enhances throughput and accuracy in single-cell analysis and printing applications.

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Last Updated: Apr 13, 2026

The Submerged Printing of Cells onto a Modified Surface Using a Continuous Flow Microspotter

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Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

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Published on: March 6, 2016

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Automated Robotic Dispensing Technique for Surface Guidance and Bioprinting of Cells

Published on: November 18, 2016

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Single-cell analysis is a rapidly growing field requiring advanced tools.
Existing methods for handling single cells have limitations in efficiency and throughput.

Purpose of the Study:

To present an improved single-cell printer with enhanced precision and throughput.
To integrate acoustophoretic focusing for better cell manipulation and analysis.

Main Methods:

Developed an improved single-cell printer utilizing acoustophoretic focusing.
Integrated an optical system for individual cell analysis prior to printing.
Used a piezoelectric transducer for cell focusing within a microfluidic channel.

Main Results:

Achieved 96% single-bead efficiency and >10 beads/minute throughput.
Demonstrated 99% focusing efficiency for beads and 86% for Raji cells to a central channel region.
Reduced bead loss from 52% ± 6% to 28% ± 1%.

Conclusions:

The improved single-cell printer offers enhanced performance for single-cell analysis.
Acoustophoretic focusing is a versatile and adaptable technique for cell manipulation in microfluidic devices.
The system enables more accurate optical analysis and reduces sample loss.