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Related Concept Videos

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

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Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Eukaryotic Transcription Inhibitors01:52

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Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
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RNA Polymerase II Accessory Proteins02:36

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Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
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Bacterial Transcription01:53

Bacterial Transcription

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RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
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Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

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Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
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Related Experiment Video

Updated: Apr 12, 2026

An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
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TAp73 transcriptionally represses BNIP3 expression.

Varvara Petrova1, Mara Mancini, Massimiliano Agostini

  • 1a Medical Research Council; Toxicology Unit; Leicester University ; Leicester , UK.

Cell Cycle (Georgetown, Tex.)
|May 8, 2015
PubMed
Summary

Tumor suppressor TAp73 directly inhibits BNIP3 gene expression. Upregulated BNIP3 in lung cancer correlates with poorer survival, suggesting TAp73

Keywords:
HIFautophagylung cancerp53p73

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Area of Science:

  • Molecular biology
  • Cancer research
  • Tumor suppressors

Background:

  • TAp73, a p53 family member, acts as a tumor suppressor.
  • TAp73 alterations can impair DNA damage response, cell cycle arrest, and apoptosis.
  • TAp73 deficiency promotes angiogenesis via hypoxia-inducible factor (HIF) signaling.

Purpose of the Study:

  • To investigate the role of TAp73 in regulating BNIP3 expression.
  • To explore the association between BNIP3 and lung cancer progression and patient survival.

Main Methods:

  • Analysis of TAp73's direct binding to the BNIP3 gene promoter.
  • Examination of human lung cancer datasets for BNIP3 expression levels.
  • Correlation analysis between BNIP3 expression and patient survival rates.

Main Results:

  • TAp73 directly suppresses the expression of BNIP3 by binding to its promoter.
  • BNIP3 is significantly upregulated in human lung cancer.
  • Elevated BNIP3 expression is directly associated with reduced survival rates in lung cancer patients.

Conclusions:

  • BNIP3 is a novel transcriptional target of TAp73.
  • TAp73's suppression of BNIP3 contributes to its tumor-suppressive function, potentially by antagonizing HIF signaling.
  • BNIP3 may serve as a prognostic biomarker in lung cancer.