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Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Related Experiment Video

Updated: Apr 12, 2026

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

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Membrane proteins--engineering, purification and crystallization. Preface

Methods in Enzymology

|May 8, 2015

Summary

No abstract available in PubMed .

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