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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
First complete genome sequence of an emerging cucumber green mottle mosaic virus isolate in north america
Rugang Li1, Yi Zheng2, Zhangjun Fei
1U.S. Department of Agriculture-Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, South Carolina, USA.
Abstract:
The complete genome sequence (6,423 nucleotides [nt]) of an emerging cucumber green mottle mosaic virus (CGMMV) isolate on cucumber in North America was determined through deep sequencing of small (sRNA) and rapid amplification of cDNA ends. The virus shares 99% nucleotide sequence identity with the Asian genotype but only 90% with the European genotype.
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