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Updated: Apr 12, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
Published on: September 2, 2021
Utilization of TALEN and CRISPR/Cas9 technologies for gene targeting and modification
Jiali Pu1, David Frescas2, Baorong Zhang3
1Department of Physiology and Biophysics, State University of New York at Buffalo, Buffalo, New York 14214, USA Department of Neurology, Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310009, China.
Abstract:
The capability to modify the genome precisely and efficiently offers an extremely useful tool for biomedical research. Recent developments in genome editing technologies such as transcription activator-like effector nuclease and the clustered regularly interspaced short palindromic repeats system have made genome modification available for a number of organisms with relative ease. Here, we introduce these genome editing techniques, compare and contrast each technical approach and discuss their potential to study the underlying mechanisms of human disease using patient-derived induced pluripotent stem cells.
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