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Development and evaluation of a real-time EBOV-L-RT-qPCR for detection of Zaire ebolavirus

Anne J Jääskeläinen1, Kirsi Moilanen2, Kirsi Aaltonen2

  • 1Helsinki University Hospital, Department of Virology and Immunology, Finland; University of Helsinki, Department of Virology, Finland.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|May 12, 2015
PubMed

Keywords:
DiagnosticsEBOVPCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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