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Programmed Synthesis by Stimuli-Responsive DNAzyme-Modified Mesoporous SiO2 Nanoparticles.

Dora Balogh1, Miguel Angel Aleman Garcia1, H Bauke Albada2

  • 1The Hebrew University of Jerusalem, Institute of Chemistry, Center for Nanoscience and Nanotechnology, Jerusalem, 91904 (Israel).

Angewandte Chemie (International Ed. in English)
|May 12, 2015
PubMed
Summary

DNAzyme-capped mesoporous silica nanoparticles act as smart containers for controlled synthesis. These responsive nanoparticles release their cargo upon specific ion or molecule triggers, enabling programmed chemical reactions and product formation.

Keywords:
DNA nanotechnologyFRETclick chemistrycontrolled releasefluorescence

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Area of Science:

  • Nanotechnology
  • Biochemistry
  • Materials Science

Background:

  • Mesoporous silica nanoparticles (MP SiO2 NPs) offer versatile platforms for drug delivery and catalysis.
  • DNAzymes are catalytic DNA molecules that can be engineered to respond to specific stimuli.
  • Stimuli-responsive materials are crucial for developing controlled and programmed synthesis methods.

Purpose of the Study:

  • To develop DNAzyme-capped mesoporous SiO2 nanoparticles (MP SiO2 NPs) as stimuli-responsive containers for programmed synthesis.
  • To demonstrate the controlled release of encapsulated molecules and subsequent "click" reactions triggered by specific ions and molecules.
  • To investigate the kinetics of product formation using fluorescence spectroscopy.

Main Methods:

  • Preparation of MP SiO2 NPs loaded with fluorescent dyes (Cy3-DBCO, Cy5-N3, Cy7-N3).
  • Capping of NPs with specific DNAzyme sequences responsive to Mg(2+), Zn(2+), and histidine.
  • Triggering NP release and subsequent "click" reactions (e.g., Cy3-Cy5, Cy3-Cy7) using specific ions and molecules.
  • Monitoring product formation and NP unloading via fluorescence spectroscopy (FRET) and kinetic modeling.

Main Results:

  • DNAzyme-capped MP SiO2 NPs successfully encapsulated and released cargo in response to specific triggers (Mg(2+), Zn(2+), histidine).
  • Programmed synthesis of fluorescent conjugates (Cy3-Cy5 and Cy3-Cy7) was achieved through triggered "click" reactions.
  • Fluorescence spectroscopy and FRET demonstrated time-dependent product formation and NP unloading.
  • A kinetic model accurately correlated with the experimental data for product formation.

Conclusions:

  • DNAzyme-capped MP SiO2 NPs serve as effective stimuli-responsive containers for programmed synthesis.
  • The system allows for precise control over chemical reactions triggered by specific biological and inorganic molecules.
  • This approach holds promise for applications in controlled synthesis, sensing, and advanced materials development.