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Lagging Strand Synthesis

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Photochemically Triggered and Autonomous Oscillatory pH-Modulated Transient Assembly/Disassembly of DNA Microdroplet

Yunlong Qin1, Yang Sung Sohn2, Xiang Li1

  • 1The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

Angewandte Chemie (International Ed. in English)
|October 8, 2024
PubMed
Summary

Researchers developed pH-responsive DNA microdroplets (MDs) that dynamically assemble and disassemble. These DNA coacervates respond to light-induced or oscillating pH changes, enabling controlled material behavior.

Keywords:
DNA nanotechnologyDNA structuresDissipative processLiquid-liquid phase separationSelf-assembly

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Area of Science:

  • Biomaterials Science
  • Supramolecular Chemistry
  • Chemical Engineering

Background:

  • Phase-separated microdroplets (MDs) are crucial for cellular organization.
  • Controlling the assembly and disassembly of synthetic MDs is key for advanced materials.
  • DNA nanotechnology offers precise control over molecular assembly.

Purpose of the Study:

  • To introduce pH-responsive DNA-based microdroplet coacervates.
  • To demonstrate light-induced and oscillatory control over MD assembly and disassembly.
  • To explore the dynamic behavior of these responsive DNA materials.

Main Methods:

  • Assembly of Y-shaped nucleic acid modules crosslinked by pH-responsive strands.
  • Utilizing a photoacid (merocyanine/spiropyran) for light-induced pH modulation (pH 6.0-4.4).
  • Employing the Landolt reaction for sustained oscillatory pH changes (pH 7.5→4.2→7.5).

Main Results:

  • Demonstrated pH-stimulated switchable and oscillatory depletion/reformation of DNA-based MDs.
  • Showcased light-induced reversible control over MDs via photochemical pH switching.
  • Observed autonomous, rhythmic assembly/disassembly of MDs synchronized with oscillating pH conditions.

Conclusions:

  • DNA-based coacervate microdroplets can be dynamically controlled by pH.
  • Photoacid and Landolt reaction provide external triggers for reversible MD behavior.
  • These findings open avenues for responsive DNA-based materials and systems.