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Updated: Apr 12, 2026

Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein GST-RhoAG17A from Epithelial Cell Lysates
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Regulation and function of P-Rex family Rac-GEFs

Heidi C E Welch1

  • 1a Signalling Programme; Babraham Institute ; Cambridge , UK.

Small Gtpases
|May 12, 2015
PubMed

Keywords:
GEFsP-Rex1P-Rex2P-Rex2bPREX1PREX2PREX2ARacRho familyaa, amino acid; BRET, bioluminescence resonance energy transfer; coIP, coimmunoprecipitation; EST, expressed sequence tag; GEF, guanine-nucleotide exchange factor; HDAC, histone deacetylase; iDHPH, isolated Dbl homology (DH) /pleckstrin homology (PH) domaguanine-nucleotide exchange factors

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