Related Experiment Video
Updated: Apr 12, 2026
12:20
Fabrication of Carbon Nanotube High-Frequency Nanoelectronic Biosensor for Sensing in High Ionic Strength Solutions
Published on: July 22, 2013
18.8K
A glucose biosensor based on partially unzipped carbon nanotubes
Huifang Hu1, Miao Feng1, Hongbing Zhan1
1College of Materials Science and Engineering, Fuzhou University, Fuzhou 350116, China.
Talanta
|May 13, 2015
Summary
A novel glucose biosensor utilizes partially unzipped carbon nanotubes (PUCNTs) to immobilize glucose oxidase (GOD), enabling mediator-free glucose detection with high sensitivity and stability. This advancement offers a promising platform for biocompatible biosensor development.
Area of Science:
- Electrochemistry
- Nanomaterials Science
- Biosensor Technology
Background:
- Glucose oxidase (GOD) is crucial for glucose biosensors.
- Carbon nanotubes (CNTs) offer excellent electrochemical properties.
- Efficient immobilization of GOD on nanomaterials is key for biosensor performance.
Purpose of the Study:
- To fabricate an amperometric glucose biosensor using partially unzipped carbon nanotubes (PUCNTs) for GOD immobilization.
- To achieve direct electron transfer between GOD and the electrode surface.
- To evaluate the biosensor's analytical performance and stability.
Main Methods:
- Synthesis of PUCNTs via chemical oxidative etching of CNTs.
- Modification of glassy carbon electrode (GCE) with PUCNTs and GOD.
- Electrochemical characterization using cyclic voltammetry.
- Amperometric detection of glucose in a mediator-free system.
Main Results:
- Successful fabrication of a PUCNT/GOD/GCE biosensor.
- Achieved fast direct electron transfer between GOD and the electrode.
- Demonstrated high sensitivity (19.50μA mM⁻¹cm⁻²), wide linear range (0-17mM), and low Michaelis-Menten constant (5.09mM).
- Exhibited excellent selectivity, resisting interference from common blood components, and good storage stability.
Conclusions:
- The developed PUCNT-based glucose biosensor offers a highly sensitive, selective, and stable platform for glucose determination.
- The facile preparation strategy provides a new avenue for fabricating advanced biocompatible biosensors.
- Direct electron transfer achieved via PUCNTs enhances biosensor efficiency in a mediator-free environment.
