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Label-free aptamer biosensor for thrombin detection based on functionalized graphene nanocomposites.
Qingqing Wang1, Zhixue Zhou2, Yanling Zhai2
1College of Chemistry, Jilin University, Changchun 130012, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Talanta
|May 13, 2015
Summary
A novel electrochemical aptasensor using graphene nanocomposites detects thrombin, a key factor in blood clots. This amplified sensor offers sensitive and label-free detection for thrombosis and hemostasis research.
Area of Science:
- Biomedical Engineering
- Electrochemistry
- Nanotechnology
Background:
- Thrombin is crucial in thrombosis and hemostasis.
- Accurate thrombin detection is vital for diagnosing and managing related conditions.
- Existing detection methods may lack sensitivity or require labels.
Purpose of the Study:
- To develop a label-free and amplified electrochemical aptasensor for thrombin detection.
- To utilize functionalized graphene nanocomposites for enhanced signal transduction.
- To establish a sensitive and reliable platform for monitoring thrombin levels.
Main Methods:
- Immobilization of thiolated aptamer (TBA15-DTT) on a gold electrode.
- Fabrication of a sandwich sensing platform using aptamer-functionalized graphene nanocomposites (rGO-TBA29).
- Electrochemical impedance spectroscopy (EIS) to measure charge-transfer resistance (Rct) changes upon thrombin binding.
Main Results:
- The aptasensor demonstrated a label-free and amplified electrochemical impedimetric response.
- An amplified charge-transfer resistance (Rct) signal was observed due to electrostatic repulsion of [Fe(CN)6](4-/3-) anions by TBA29.
- A linear relationship between Rct increase and thrombin concentration was established from 0.3 to 50 nM.
- A low detection limit of 0.01 nM for thrombin was achieved.
Conclusions:
- The developed rGO-AuNPs based aptasensor provides a sensitive and amplified method for thrombin detection.
- The platform's design allows for signal amplification through electrostatic interactions.
- Graphene's versatility enables further modifications for diverse detection strategies and applications in biosensing.
