Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.7K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.7K
IR Spectrometers01:25

IR Spectrometers

3.5K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
3.5K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.8K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.9K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.9K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

5.5K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
5.5K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

1.0K
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrasensitive Chemical Detection Using Integrating Cavity-Enhanced Raman Spectroscopy.

Analytical chemistry·2026
Same author

Stress-induced Rab11a-exosomes induce amphiregulin-mediated cetuximab resistance in colorectal cancer.

Journal of extracellular vesicles·2024
Same author

Can surgical skills be taught using technological advances online? A comparative study of online and face-to-face surgical skills training.

Surgical endoscopy·2022
Same author

Glutamine deprivation alters the origin and function of cancer cell exosomes.

The EMBO journal·2020
Same author

Integrating cavity device for measuring the optical backscattering coefficient in a fluid.

Applied optics·2018
Same author

Investigating the collection and assessment of patient-reported outcome data amongst unplanned surgical hospital admissions: a feasibility study.

Pilot and feasibility studies·2016
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
15:04

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Published on: May 18, 2011

13.6K

Diffuse reflecting material for integrating cavity spectroscopy, including ring-down spectroscopy.

Michael T Cone, Joseph A Musser, Eleonora Figueroa

    Applied Optics
    |May 14, 2015
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a highly reflective fumed silica material for integrating cavities. This novel material enables precise absorption spectroscopy using integrating cavity ring-down spectroscopy.

    More Related Videos

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    13.5K
    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks
    10:31

    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

    Published on: May 8, 2015

    14.3K

    Related Experiment Videos

    Last Updated: Apr 12, 2026

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
    15:04

    Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

    Published on: May 18, 2011

    13.6K
    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    13.5K
    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks
    10:31

    Diffuse Reflectance Infrared Spectroscopic Identification of Dispersant/Particle Bonding Mechanisms in Functional Inks

    Published on: May 8, 2015

    14.3K

    Area of Science:

    • Materials Science
    • Optical Engineering
    • Spectroscopy

    Background:

    • High-reflectivity surfaces are crucial for optical cavities.
    • Developing materials with near-perfect diffuse reflectivity is challenging.
    • Integrating cavities enhance light-matter interaction for spectroscopy.

    Purpose of the Study:

    • To develop a novel diffuse reflecting material with ultra-high reflectivity.
    • To characterize the material's optical properties and Lambertian behavior.
    • To demonstrate its application in advanced spectroscopic techniques.

    Main Methods:

    • Synthesis and characterization of high-purity fumed silica nanoparticles (approx. 40 nm).
    • Measurement of diffuse reflectivity at 532 nm and 266 nm.
    • Measurement of effective wall time within integrating cavities.
    • Development of integrating cavity ring-down spectroscopy (ICRDS).

    Main Results:

    • Achieved reflectivity values of 0.99919 at 532 nm and 0.99686 at 266 nm.
    • Demonstrated near-Lambertian surface behavior.
    • Measured effective wall time on the order of picoseconds.
    • Successfully implemented ICRDS for absorption spectroscopy.

    Conclusions:

    • The developed fumed silica material offers exceptional diffuse reflectivity.
    • This material is suitable for constructing high-performance integrating cavities.
    • ICRDS provides a sensitive method for absorption spectroscopy within these cavities.