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Updated: Apr 12, 2026

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

Published on: June 4, 2019

Improving the ostrich genome assembly using optical mapping data.

Jilin Zhang¹, Cai Li², Qi Zhou³

¹China National GeneBank, BGI-Shenzhen, Shenzhen,, 518083 China.

|May 14, 2015

Summary

This summary is machine-generated.

Optical mapping significantly improved ostrich genome assembly, extending scaffolds fivefold. This advancement aids avian comparative genomics and chromosome evolution studies by providing a more contiguous genome sequence.

Keywords:

Genome assembly Optical mapping Ostrich

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Last Updated: Apr 12, 2026

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

Published on: June 4, 2019

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization

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Published on: June 28, 2012

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

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Published on: November 1, 2014

Area of Science:

Genomics
Comparative Genomics
Avian Phylogenomics

Background:

Ostrich meat is a healthy red meat with significant global production.
The ostrich genome was initially sequenced for avian phylogenomics.
The first ostrich genome assembly had limitations in scaffold contiguity (N50 of 3.59 Mb).

Purpose of the Study:

To improve the ostrich genome assembly using optical mapping (OM).
To enhance scaffold contiguity for advanced genomic analyses, including chromosome-level comparisons.

Main Methods:

Generated optical mapping data by digesting ostrich DNA with KpnI.
Assembled OM data with existing Illumina-based assembly for sequence extension.
Integrated OM data with fluorescence in situ hybridization (FISH) markers.

Main Results:

Achieved a 5-fold increase in scaffold N50 to 17.71 Mb.
Reduced the number of scaffolds covering 90% of the genome from 414 to 75.
Successfully recovered the pseudoautosomal region (PAR) on the Z chromosome.

Conclusions:

Optical mapping data significantly enhanced ostrich genome assembly quality.
Improved assembly facilitates studies on avian chromosome evolution.
This strategy is applicable to other genome sequencing projects for better assemblies.