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Updated: Apr 12, 2026
Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP
Published on: September 8, 2023
Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification
Nelson R Salinas1, Damon P Little2
1Cullman Program for Molecular Systematics, The New York Botanical Garden, Bronx, NY 10458, USA ; The Graduate Center, The City University of New York, New York, NY 10016, USA.
Abstract:
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole genome sequences of Staphylococcus. eLAMP correctly predicted that the two tested primer sets would amplify from S. aureus genomes and not amplify from other Staphylococcus species. Open source (GNU Public License) PERL scripts are available for download from the New York Botanical Garden's website.
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