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Published on: May 14, 2012
Adoptive transfer of transforming growth factor-ß1-induced CD4+CD25+ regulatory T cells prevents immune
Tian Qiu1, Yincheng Teng1, Yudong Wang2
1Department of Obstetrics and Gynecology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, 600 Yishan Road, Shanghai 200233, China.
The effects of adoptive transfer of transforming growth factor (TGF)-ß1-induced regulatory T (Treg) cells in preventing spontaneous abortion in mice were investigated. CD4+CD25- cells were isolated from the spleens of pregnant CBA/J mice and induced into Treg cells positive for CD4, CD25 and forkhead box P3 (FOXP3) ex vivo using interleukin (IL)-2 and TGF-ß1. CBA/J mice were mated with DBA/2J mice to establish a model of spontaneous abortion and, on the first day of pregnancy, mice were injected intravenously with 2 × 105 either freshly isolated Treg cells or those induced with TGF-ß1. After 14 days, the surviving and reabsorbed fetuses in both groups were counted, and serum cytokine concentrations were measured by ELISA. Adoptive transfer of CD4+CD25+ or TGF-ß1-induced Treg cells significantly reduced the fetal resorption rate, increased serum IL-10 and TGF-ß1 concentrations and decreased interferon-? levels. In conclusion, the results of the present study indicate that adoptive transfer of TGF-ß1-induced Treg cells prevents spontaneous abortion in mice by increasing the secretion of T helper (Th) 2 cytokines and decreasing the secretion of Th1 cytokines.
The effects of adoptive transfer of transforming growth factor (TGF)-ß1-induced regulatory T (Treg) cells in preventing spontaneous abortion in mice were investigated. CD4+CD25- cells were isolated from the spleens of pregnant CBA/J mice and induced into Treg cells positive for CD4, CD25 and forkhead box P3 (FOXP3) ex vivo using interleukin (IL)-2 and TGF-ß1. CBA/J mice were mated with DBA/2J mice to establish a model of spontaneous abortion and, on the first day of pregnancy, mice were injected intravenously with 2 × 105 either freshly isolated Treg cells or those induced with TGF-ß1. After 14 days, the surviving and reabsorbed fetuses in both groups were counted, and serum cytokine concentrations were measured by ELISA. Adoptive transfer of CD4+CD25+ or TGF-ß1-induced Treg cells significantly reduced the fetal resorption rate, increased serum IL-10 and TGF-ß1 concentrations and decreased interferon-? levels. In conclusion, the results of the present study indicate that adoptive transfer of TGF-ß1-induced Treg cells prevents spontaneous abortion in mice by increasing the secretion of T helper (Th) 2 cytokines and decreasing the secretion of Th1 cytokines.
