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C-Jun recruits the NSL complex to regulate its target gene expression by modulating H4K16 acetylation and promoting

Yan Liu1, Yuehong Long1, Zhaobin Xing1

  • 1College of Life Sciences, Hebei United University, Tangshan, China.

Oncotarget
|May 15, 2015
PubMed

Insights

The non-specific lethal (NSL) complex acts as a c-Jun co-activator, promoting gene transcription by catalyzing H4K16 acetylation and releasing the NuRD complex at target genes.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Epigenetics

Background:

  • The proto-oncogene c-Jun is crucial for cell proliferation, differentiation, and apoptosis.
  • The males absent on the first (MOF)-containing non-specific lethal (NSL) complex is known to positively regulate gene expression.
  • The precise biological functions of the NSL complex remain largely uncharacterized.

Purpose of the Study:

  • To elucidate the role of the NSL complex in c-Jun-mediated gene expression.
  • To identify novel mechanisms regulating c-Jun activation.
  • To expand the understanding of NSL complex functions in gene regulation.

Main Methods:

  • Investigating the recruitment of the NSL complex to c-Jun target genes.
  • Analyzing the impact of NSL complex on H4K16 acetylation.
  • Assessing the effect of the NSL complex on NuRD complex release.

Main Results:

  • c-Jun recruits the NSL complex to its target genes upon activation.
  • The NSL complex catalyzes H4K16 acetylation at c-Jun target genes, enhancing transcription.
  • The NSL complex facilitates the release of the repressive NuRD complex from c-Jun target genes.

Conclusions:

  • The NSL complex functions as a c-Jun co-activator.
  • NSL complex-mediated H4K16 acetylation and NuRD complex release are key mechanisms in c-Jun target gene activation.
  • This study reveals a new regulatory pathway for c-Jun activation and expands the known functions of the NSL complex.
Keywords:
C-JunH4K16 acetylationNSL complexNuRD complexphosphorylation

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