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Purification of human serum amyloid P component (SAP) by calcium affinity chromatography
T Mantovaara1, H Pertoft, J Porath
1Institute of Biochemistry, University of Uppsala, Sweden.
Biotechnology and Applied Biochemistry
|December 1, 1989
Abstract:
Serum amyloid P component (SAP) has been purified from human serum by means of immobilized metal ion affinity chromatography (IMAC). It was selectively concentrated on carboxymethylated aspartic acid agarose (CM-Asp-agarose) loaded with calcium and, employing very mild conditions, purified to electrophoretical and immunological homogeneity in a single step amounting to about 1900-fold purification. As a purification method our procedure thus compares well with bio-specific affinity chromatography.