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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.8K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

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When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...
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Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

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Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
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Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

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Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
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Related Experiment Video

Updated: Apr 12, 2026

Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
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Profiling planktonic biomass using element-specific, multicomponent nuclear magnetic resonance spectroscopy.

Takanori Komatsu1,2, Toshiya Kobayashi2, Minoru Hatanaka3

  • 1†RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan.

Environmental Science & Technology
|May 15, 2015
PubMed
Summary

This study introduces advanced solid-state nuclear magnetic resonance (ssNMR) for plankton chemical speciation. It reveals how Euglena gracilis utilizes arginine and polyphosphate reserves in response to ammonium, impacting elemental cycles.

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Area of Science:

  • Biogeochemistry
  • Microbial Ecology
  • Analytical Chemistry

Background:

  • Planktonic metabolism is vital for Earth's elemental cycles.
  • Understanding plankton's role requires knowledge of chemical speciation and elemental stoichiometry.

Purpose of the Study:

  • To propose a multicomponent solid-state nuclear magnetic resonance (ssNMR) approach for cellular chemical speciation.
  • To characterize cellular components of Euglena gracilis using advanced NMR techniques.
  • To demonstrate the method's utility in profiling and comparing cellular components under varying nutrient conditions.

Main Methods:

  • Utilized (13)C and (15)N-labeled Euglena gracilis.
  • Applied 3D dipolar-assisted rotational resonance (DARR).
  • Employed double-cross-polarization (1)H-(13)C correlation spectroscopy and (1)H-(13)C solid-state heteronuclear single quantum correlation spectroscopy.

Main Results:

  • Ammonium availability induced paramylon degradation and amination in E. gracilis.
  • Arginine accumulation was observed as a nitrogen reserve, with ammonium replaced via the arginine dihydrolase pathway.
  • (15)N and (31)P ssNMR confirmed arginine and polyphosphate accumulation, respectively.

Conclusions:

  • The developed ssNMR technique enables detailed chemical speciation of planktonic cellular components.
  • This method provides insights into nutrient assimilation and storage strategies in protists like E. gracilis.
  • The technique contributes to environmental research by offering detailed chemical property information.