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Published on: May 10, 2016
Protective Effects of Pyridoxamine Against UVC-induced Programmed Cell Death in HaCaT Cells
Shou-Cheng Wang1, Hong-Xue Ji2, Chieh-Lun Hsiao2
1Department of Medical Imaging and Radiological Sciences, Central-Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, Taichung Armed Forces General Hospital, Taichung, Taiwan, R.O.C.
Background:
Exposure to ultraviolet (UV) light is closely related to human diseases, such as skin cancer, due to irreversible injuries to the skin cells. The UV-induced DNA damage and programmed cell death are important determinants for skin carcinogenesis. The aim of the present study was to investigate the anti-ultraviolet-C (UVC) effects of pyridoxamine in human keratinocyte HaCaT cells and its mechanisms of action.
Results:
UVC-induced programmed cell death in HaCaT cells was abrogated by treated the cells immediately after UVC irradiation with 40, 80 and 160 μM of pyridoxamine. Monitoring the UVC-induced-specific reactive oxygen species, we found that 20, 40, 80 and 160 μM of pyridoxamine was also effective in suppressing the induction of reactive oxygen species by UVC.
Conclusion:
Overall, our results provided evidence showing that pyridoxamine was effective in protecting HaCaT cells from UVC-induced programmed cell death and may be a potential anti-UVC agent in life and clinical practice.
