Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

12.6K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.6K
Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct neural processes link speech planning and execution.

bioRxiv : the preprint server for biology·2026
Same author

Prenatal e-cigarette exposure induces region- and cell type-specific metabolic and developmental reprogramming.

bioRxiv : the preprint server for biology·2026
Same author

Astrocytic D1 Dopamine-Signaling Regulates Synaptic Remodeling and Cocaine Seeking.

bioRxiv : the preprint server for biology·2026
Same author

Palliative Care or Hospice? Flipping the Classroom for 1st Year Pre-clinical Medical Students With Interactive Online Content.

The American journal of hospice & palliative care·2026
Same author

Prenatal THC exposure disrupts mitochondrial respiratory gene programs and medium spiny neuron maturation trajectories in the nucleus accumbens.

bioRxiv : the preprint server for biology·2026
Same author

Refinement of Nucleus Accumbens Neuronal Dynamics during Cocaine Self-Administration Training.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026

Related Experiment Video

Updated: Apr 12, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

8.4K

Transcriptomic profiling of rat liver samples in a comprehensive study design by RNA-Seq.

Binsheng Gong1, Charles Wang2, Zhenqiang Su1

  • 1Division of Bioinformatics and Biostatistics, National Center for Toxicological Research, Food and Drug Administration , Jefferson, Arkansas 72079, USA.

Scientific Data
|May 16, 2015
PubMed
Summary

The US FDA

More Related Videos

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

541
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

8.0K

Related Experiment Videos

Last Updated: Apr 12, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

8.4K
Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes
05:07

Rup (RNA-seq Usability Assessment Pipeline) - Quality Control for Bulk RNA-seq Experiments in Eukaryotes

Published on: November 7, 2025

541
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

8.0K

Area of Science:

  • Toxicogenomics and Transcriptome Profiling
  • Chemical Safety Assessment
  • Next-Generation Sequencing Applications

Background:

  • RNA sequencing (RNA-Seq) offers comprehensive transcriptome analysis, including gene expression and alternative splicing.
  • Evaluating RNA-Seq's utility in safety assessment and understanding toxicity mechanisms is crucial.
  • The US Food and Drug Administration (FDA)-led Sequencing Quality Control (SEQC) project, also known as MAQC-III, is a key initiative.

Purpose of the Study:

  • To assess the utility of RNA-Seq for safety assessment and toxicity mechanism elucidation.
  • To characterize transcriptome-wide changes induced by chemical perturbations.
  • To establish a comprehensive toxicogenomics dataset for research.

Main Methods:

  • Profiling of rat liver transcriptomes using paired-end 100 bp RNA-Seq.
  • Treatment of rats with 27 chemicals representing diverse chemogenomic modes of action.
  • High-throughput sequencing performed on Illumina HiScanSQ and/or HiSeq 2000 platforms.

Main Results:

  • Generated a large toxicogenomics dataset from chemical-treated rat liver samples.
  • Demonstrated varying degrees of transcriptional response to different chemicals.
  • Highlighted the capability of RNA-Seq to detect complex transcriptomic alterations, such as alternative splicing.

Conclusions:

  • RNA-Seq is a valuable tool for comprehensive transcriptome profiling in safety assessment.
  • The generated dataset serves as a significant resource for studying chemical-induced toxicity mechanisms.
  • Further analysis of the dataset can reveal detailed transcriptomic changes, including alternative splicing events.