Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

2.0K
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
2.0K
Regulated mRNA Transport02:22

Regulated mRNA Transport

3.5K
3.5K
Regulated mRNA Transport02:22

Regulated mRNA Transport

7.3K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
7.3K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.6K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.6K
Translational Regulation01:29

Translational Regulation

859
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
859

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic and therapeutic applications of the glycan biomarker H3N2b in GM1 Gangliosidosis.

Molecular genetics and metabolism·2026
Same author

The sphingosine-1-phosphate pathway is differentially activated in human gestational tissues.

Journal of the Endocrine Society·2026
Same author

Small-molecule correlates of infection precede infection diagnosis in breast implant reconstruction patients.

The Journal of clinical investigation·2025
Same author

Secondary accumulation of lyso-platelet activating factors in lysosomal storage diseases.

Molecular genetics and metabolism·2025
Same author

The Sphingosine-1-phosphate pathway is differentially activated in human gestational tissues.

bioRxiv : the preprint server for biology·2025
Same author

Accumulation of alkyl-lysophosphatidylcholines in Niemann-Pick disease type C1.

Journal of lipid research·2024
Same journal

Insulin resistance and type 2 diabetes as allostatic responses to chronic nutrient excess.

Cell metabolism·2026
Same journal

Microbiota and immunotherapy: The birth of a new paradigm?

Cell metabolism·2026
Same journal

Glycerol activates tissue-nonspecific alkaline phosphatase for temperature and bone homeostasis.

Cell metabolism·2026
Same journal

Jack-of-a-new-trade: Itaconate targets the PPP/G6PD to control lung cancer.

Cell metabolism·2026
Same journal

A liver phosphatase reprograms gut stem cells to drive hyperglycemia.

Cell metabolism·2026
Same journal

GLP-1 and MASH: When weight loss isn't the whole story.

Cell metabolism·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

14.3K

snoRNA U17 regulates cellular cholesterol trafficking.

Sarah Jinn1, Katrina A Brandis1, Aileen Ren1

  • 1Diabetic Cardiovascular Disease Center and Department of Medicine, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.

Cell Metabolism
|May 19, 2015
PubMed
Summary
This summary is machine-generated.

A novel U17 snoRNA pathway regulates cholesterol trafficking and steroid hormone synthesis by controlling HUMMR mRNA levels. This discovery reveals a new role for U17 snoRNA in cellular cholesterol metabolism and gonadal development.

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.5K
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K

Related Experiment Videos

Last Updated: Apr 12, 2026

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
08:45

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Published on: November 17, 2018

14.3K
A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

2.5K
Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection
07:35

Analysis of Spliceosomal snRNA Localization in Human Hela Cells Using Microinjection

Published on: August 6, 2019

6.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cholesterol is essential for mammalian cell function and steroid hormone production.
  • Intracellular cholesterol trafficking is critical for cellular homeostasis.
  • The function of U17 snoRNA beyond ribosomal processing was unknown.

Purpose of the Study:

  • To identify novel regulators of intracellular cholesterol trafficking.
  • To elucidate the function of the U17 snoRNA.
  • To investigate the role of the U17 snoRNA-HUMMR pathway in steroidogenesis.

Main Methods:

  • Loss-of-function genetic screen in Chinese hamster ovary cells.
  • Expression profiling to identify U17 snoRNA targets.
  • Analysis of ER-mitochondrial contacts and cholesterol esterification.
  • In vivo studies in steroidogenic tissues.

Main Results:

  • A U17 snoRNA-deficient mutant exhibited defects in cholesterol trafficking.
  • Hypoxia-upregulated mitochondrial movement regulator (HUMMR) mRNA was identified as a U17 snoRNA target.
  • Upregulation of HUMMR promoted ER-mitochondrial contacts and altered cholesterol metabolism.
  • The U17 snoRNA-HUMMR pathway regulates mitochondrial steroid synthesis in vivo.

Conclusions:

  • U17 snoRNA negatively regulates HUMMR mRNA.
  • The U17 snoRNA-HUMMR pathway plays a physiological role in cholesterol trafficking and steroidogenesis.
  • This pathway is developmentally regulated in gonadal tissues, suggesting a role in maturation.