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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Updated: Apr 12, 2026

Fluorescence Imaging with One-nanometer Accuracy FIONA

Fluorescence Imaging with One-nanometer Accuracy FIONA

Published on: September 26, 2014

STED nanoscopy with fluorescent quantum dots.

Janina Hanne¹, Henning J Falk¹, Frederik Görlitz¹

¹German Cancer Research Center (DKFZ), Optical Nanoscopy Division, Im Neuenheimer Feld 280, Heidelberg 69120, Germany.

Nature Communications

|May 19, 2015

Summary

This summary is machine-generated.

Researchers successfully used quantum dots for STED nanoscopy, achieving ~50 nm resolution. This breakthrough overcomes previous limitations, enabling advanced imaging of biological structures with high photostability and reduced blinking.

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Last Updated: Apr 12, 2026

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Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Area of Science:

Optical Microscopy
Nanotechnology
Biophysics

Background:

Quantum dots are popular molecular labels but have been incompatible with STED nanoscopy due to spectral overlap.
Broad excitation spectra of quantum dots interfere with the STED process, preventing super-resolution.

Purpose of the Study:

To investigate the feasibility of using quantum dots as fluorescent probes in STED nanoscopy.
To overcome the spectral limitations hindering quantum dot application in STED.

Main Methods:

Utilized red-emitting commercially available quantum dots.
Employed a 775 nm STED laser for nanoscopy.
Demonstrated imaging of single quantum dots and labeled vimentin filaments.

Main Results:

Achieved super-resolution STED nanoscopy with quantum dots using a 775 nm laser.
Demonstrated a resolution of approximately 50 nm for single quantum dots.
Showcased sub-diffraction imaging of quantum-dot-labeled vimentin filaments in fibroblasts.
Observed high photostability enabling over 1,000 frames and reduced blinking.

Conclusions:

Quantum dot STED nanoscopy is now feasible, expanding STED applications.
The high photostability and reduced blinking of quantum dots are advantageous for imaging.
This technique holds promise for extended time-lapse super-resolution imaging.