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Updated: Apr 12, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
Published on: March 13, 2018
Real-Time LAMP for Chalara fraxinea Diagnosis
Jenny Tomlinson1, Neil Boonham
1The Food and Environment Research Agency, Sand Hutton, York, YO41 1LZ, UK, jenny.tomlinson@fera.gsi.gov.uk.
Abstract:
Chalara fraxinea is the causal agent of ash dieback, a disease affecting Fraxinus excelsior and F. angustifolia across Europe. Loop-mediated isothermal amplification (LAMP) is a rapid, DNA-based method which can be used for specific detection of plant pathogens in infected material. The combination of a rapid LAMP assay for C. fraxinea with a simple sample preparation method in a user-friendly kit format raises the potential for testing to be carried out away from conventional laboratory facilities, to expedite measure to manage this damaging disease.
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