Related Experiment Video

Updated: Apr 12, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.6K

The SWI/SNF chromatin remodelling complex: Its role in maintaining genome stability and preventing tumourigenesis

Peter M Brownlee1, Cornelia Meisenberg1, Jessica A Downs1

  • 1Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton BN1 9RQ, UK.

DNA Repair
|May 19, 2015
PubMed

Insights

Mutations in SWI/SNF chromatin remodeling complexes are common in human cancers. These complexes have transcription-independent roles in DNA repair and genome stability, crucial for tumor suppression.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • SWI/SNF chromatin remodeling complexes (BAF and PBAF) are frequently mutated in human cancers.
  • These complexes are known regulators of gene transcription.
  • Emerging evidence suggests roles beyond transcription.

Purpose of the Study:

  • To review transcription-independent functions of SWI/SNF complexes.
  • To discuss the relevance of these functions to cancer development.

Main Methods:

  • Literature review of recent studies on SWI/SNF complexes.
  • Analysis of experimental data linking SWI/SNF to DNA damage response and sister chromatid cohesion.

Main Results:

  • SWI/SNF complexes play critical roles in DNA damage responses.
  • These complexes are essential for maintaining sister chromatid cohesion.
  • These functions impact genome stability.

Conclusions:

  • Transcription-independent functions of SWI/SNF complexes are vital for genome stability.
  • Dysregulation of these functions may contribute to tumorigenesis.
Keywords:
BAF180BRG1Chromatin remodelling complexCohesionDNA damage induced transcriptional repressionGenome instabilityPBAFSWI/SNFTumourigenesis

More Related Videos

CD Spectroscopy to Study DNA-Protein Interactions06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.9K
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

10.4K

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.7K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.3K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
10.1K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.9K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.6K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.6K