Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Colloidal State01:29

The Colloidal State

163
The formation of a colloidal system is exemplified by an aqueous solution containing Cl− ions is introduced to another containing Ag+ ions, resulting in the precipitation of solid AgCl as extremely tiny crystals. Instead of settling out as a filterable precipitate, these crystals remain suspended in the liquid, showcasing a colloidal system.A colloidal system involves colloidal particles within the approximate range of 1 to 1000 nm in at least one dimension, dispersed in a medium called...
163
Protein Folding01:25

Protein Folding

12.7K
Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
12.7K
Protein Folding01:22

Protein Folding

131.1K
Overview
131.1K
Colloids03:22

Colloids

22.2K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
22.2K
Recrystallization: Solid–Solution Equilibria01:10

Recrystallization: Solid–Solution Equilibria

4.7K
Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...
4.7K
Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

987
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
987

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Call For Papers: Molecular Understanding and Formulation Design for Peptide Delivery.

Molecular pharmaceutics·2026
Same author

Storage Buffer Composition Impacts Internal Structure, Freeze-Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles.

ACS nano·2026
Same author

Hydrogel Fiber Endomicroscopy.

bioRxiv : the preprint server for biology·2026
Same author

Sustained Delivery of Chondrogenic Molecules Using Sugar Glass Nanoparticle-Integrated Fibrous Scaffolds for Cartilage Tissue Engineering.

Regenerative engineering and translational medicine·2026
Same author

Characterization of Peptide-Preservative Interaction by AlphaFold, Molecular Dynamics Simulation, and NMR Spectroscopy.

Molecular pharmaceutics·2025
Same author

Shear and Dilatational Rheology and Interfacial Structure of a Monoclonal Antibody Adsorbed at the Air-Liquid Interface.

Langmuir : the ACS journal of surfaces and colloids·2025

Related Experiment Video

Updated: Apr 12, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.6K

Stabilization of proteins in solid form.

Marcus T Cicerone1, Michael J Pikal2, Ken K Qian3

  • 1Materials Measurement Lab, National Institute of Standards and Technology, Gaithersburg, MD 20899-8543, USA; Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.

Advanced Drug Delivery Reviews
|May 19, 2015
PubMed
Summary
This summary is machine-generated.

Ensuring protein stability in biopharmaceuticals is crucial. This study reviews characterization methods and proposes physical principles for creating stable solid protein delivery vehicles, focusing on strong protein-matrix coupling.

Keywords:
AggregationDynamic stabilizationLyophilizationProtein stabilityWater substitution

More Related Videos

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

18.0K
Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.8K

Related Experiment Videos

Last Updated: Apr 12, 2026

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
07:22

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Published on: February 11, 2019

29.6K
Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

18.0K
Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

12.8K

Area of Science:

  • Biopharmaceutical development
  • Protein formulation science
  • Materials science

Background:

  • Protein aggregation and degradation in biopharmaceutical products is a significant concern.
  • Delivering stable, active protein formulations is critical for therapeutic efficacy.
  • Current characterization methods for solid protein dosage forms aim to predict stability but require critical evaluation.

Purpose of the Study:

  • To review and critically evaluate characterization approaches for solid protein dosage forms.
  • To revise hypotheses regarding the correlation between material properties and protein stability.
  • To establish physical principles for designing stable solid protein delivery vehicles.

Main Methods:

  • Literature review of characterization approaches for solid protein dosage forms.
  • Critical evaluation of correlations between measured material properties and protein stability outcomes.
  • Analysis of physical principles governing protein stability in solid matrices.

Main Results:

  • Existing characterization metrics for protein stability require re-evaluation based on their predictive power.
  • Hypotheses linking material properties to protein stability need revision.
  • Proteins should be strongly coupled (e.g., via H-bonds) to a phase-homogeneous matrix with suppressed beta relaxation for optimal stability.

Conclusions:

  • A framework for reliable characterization of solid protein forms concerning stability is provided.
  • Simple physical principles are identified as necessary for stable protein loads in solid delivery vehicles.
  • Strong protein-matrix coupling and suppressed matrix relaxation are key to preventing protein aggregation and degradation.