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Development of a Custom-Designed, Pan Genomic DNA Microarray to Characterize Strain-Level Diversity among Cronobacter

Ben Davies Tall1, Jayanthi Gangiredla1, Gopal R Gopinath1

  • 1Center of Food Safety and Applied Nutrition, U. S. Food and Drug Administration , Laurel, MD , USA.

Frontiers in Pediatrics
|May 19, 2015
PubMed

Insights

A new DNA microarray effectively distinguishes Cronobacter species, revealing significant genomic diversity within this foodborne pathogen genus. This tool aids in tracking Cronobacter for enhanced food safety and clinical surveillance.

Area of Science:

  • Microbiology
  • Genomics
  • Food Safety

Background:

  • Cronobacter species cause severe infections, particularly in neonates, with limited established genomics-based epidemiology.
  • Understanding the genetic diversity of Cronobacter is crucial for developing effective countermeasures against this foodborne pathogen.

Purpose of the Study:

  • To develop and evaluate a pan-genomic DNA microarray for genotyping Cronobacter species.
  • To assess the utility of the microarray for distinguishing species, identifying outbreak isolates, and understanding genomic diversity.

Main Methods:

  • A DNA microarray was designed with 19,287 genes from 15 Cronobacter genomes, plasmids, and virulence factors.
  • The microarray was used to genotype 126 diverse Cronobacter and related isolates.

Main Results:

  • The microarray successfully differentiated the seven Cronobacter species from each other and non-Cronobacter species.
  • Isolates clustered based on genomic diversity within each species, highlighting significant genetic variation.
  • The study confirmed the phylogenetic divergence of the genus and the genomic diversity among its members.

Conclusions:

  • The developed Cronobacter DNA microarray is a powerful tool for genomics research and epidemiological surveillance.
  • This platform supports food safety, environmental, and clinical surveillance efforts against Cronobacter infections.
  • Further research using this platform is essential for developing future countermeasures against this significant foodborne pathogen.

Keywords:
Cronobactergenomic diversitymicroarraypan genomicphylogenic divergence

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