Preclinical mouse models of osteosarcoma

Area of Science:

• Oncology
• Genetics
• Animal Models

Background:

• Osteosarcoma is the most common primary bone tumor, predominantly affecting children.
• Low survival rates are associated with osteosarcoma, especially when metastatic disease is present.
• Mouse models are crucial for understanding osteosarcoma tumorigenesis, metastasis, and evaluating therapeutic strategies.

Purpose of the Study:

• To review existing mouse models for osteosarcoma research.
• To provide methodologies for osteosarcoma research using mouse models.

Main Methods:

• Isolation of cell lines from genetically engineered mouse models (GEMMs).
• Gene modification techniques for osteosarcoma research.
• Tumor cell injection methods in mice.
• Imaging techniques for osteosarcoma assessment in vivo.

Main Results:

• This chapter provides a comprehensive overview of available osteosarcoma mouse models.
• Detailed protocols are presented for cell line isolation, genetic manipulation, tumor induction, and imaging.

Conclusions:

• Mouse models are indispensable tools for advancing osteosarcoma research.
• The methodologies described facilitate the study of osteosarcoma mechanisms and the development of novel therapies.