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Related Experiment Videos

Fibronectin gene expression during limb cartilage differentiation.

W M Kulyk1, W B Upholt, R A Kosher

  • 1Department of Anatomy, University of Connecticut Health Center, Farmington 06032.

Development (Cambridge, England)
|July 1, 1989
PubMed
Summary

Fibronectin (FN) expression significantly increases during limb bud cell condensation and cartilage formation. This study quantifies FN gene and protein levels during chondrogenesis in vitro and in vivo.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Limb cartilage differentiation involves cellular condensation.
  • Fibronectin (FN) is proposed to regulate this condensation and chondrogenesis.

Purpose of the Study:

  • To quantitatively assess Fibronectin (FN) gene expression during chondrogenesis.
  • To investigate the role of FN in cellular condensation and cartilage matrix deposition.

Main Methods:

  • Quantitative analysis of FN mRNA levels in limb mesenchymal cell micromass cultures.
  • Measurement of relative FN synthesis rates using pulse-labeling and immunoprecipitation.
  • In vivo analysis of FN expression in developing limb buds.

Main Results:

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  • FN mRNA levels and synthesis rates increase 4-5 fold during cell condensation and chondrogenesis in vitro.
  • These levels decline as cartilage matrix accumulates.
  • Similar high FN expression is observed in vivo at the onset of chondrogenesis in limb bud core regions.

Conclusions:

  • Fibronectin (FN) expression is upregulated during the critical cellular condensation phase of chondrogenesis.
  • FN plays a significant role in regulating early cartilage development.