Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

61.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
61.8K
RNA Splicing01:32

RNA Splicing

20.5K
20.5K
Alternative RNA Splicing02:18

Alternative RNA Splicing

27.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
27.1K
Alternative RNA Splicing02:18

Alternative RNA Splicing

5.6K
5.6K
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

7.4K
7.4K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.2K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CPEB4 deficiency promotes vasculogenic mimicry and resistance to anti-angiogenic therapy in hepatocellular carcinoma.

Gut·2026
Same author

Transcription factor cooperativity at a GATA3 tandem DNA sequence determines oncogenic enhancer-mediated activation.

Cell reports·2026
Same author

A crosstalk between tumor cells and adipocytes facilitates tumor cell migration and invasion.

International journal of biological sciences·2026
Same author

A roadmap for systematic humanization of a chimeric antigen receptor: preclinical validation of a humanized CD22 scFv as a model.

Experimental hematology·2026
Same author

Engineered Mycoplasma pneumoniae targeting dual-species bacterial biofilms: a novel strategy against infections.

NPJ biofilms and microbiomes·2025
Same author

CAR-T cells targeting CCR9 and CD1a for the treatment of T cell acute lymphoblastic leukemia.

Journal of hematology & oncology·2025
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Apr 12, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K

Splicing of a non-coding antisense transcript controls LEF1 gene expression.

Manuel Beltran1, Estel Aparicio-Prat2, Rocco Mazzolini2

  • 1Programa de Recerca en Càncer, Institut Hospital del Mar d'Investigacions Mèdiques, 08003 Barcelona, Spain UCL Cancer Institute, University College London, London, WC1E6BT, UK.

Nucleic Acids Research
|May 21, 2015
PubMed
Summary
This summary is machine-generated.

Natural antisense transcripts (NATs) regulate LEF1 gene expression. An unspliced LEF1 NAT down-regulates transcription by interacting with the promoter and facilitating PRC2 binding, while the spliced form counteracts this effect.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.6K

Related Experiment Videos

Last Updated: Apr 12, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.4K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.7K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

6.6K

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Non-coding RNA

Background:

  • LEF1 transcription factor is crucial for cellular processes.
  • Natural antisense transcripts (NATs) are emerging regulators of gene expression.
  • The role of LEF1 NAT in controlling LEF1 expression is not fully understood.

Purpose of the Study:

  • To investigate the function of the natural antisense transcript (NAT) of the LEF1 gene.
  • To elucidate the mechanism by which LEF1 NAT regulates LEF1 transcription factor expression.
  • To determine the role of alternative splicing of LEF1 NAT in its regulatory function.

Main Methods:

  • Analysis of LEF1 NAT and LEF1 mRNA expression in different cell lines.
  • Investigating the interaction of LEF1 NAT with the LEF1 promoter.
  • Assessing the role of PRC2 complex and histone modifications in LEF1 regulation.
  • Studying the effect of spliced vs. unspliced LEF1 NAT in trans.

Main Results:

  • A natural antisense transcript (NAT) is transcribed from the LEF1 gene's first intron.
  • In mesenchymal cells, NAT undergoes splicing, while in intermediate cells, it remains unspliced.
  • Unspliced LEF1 NAT down-regulates LEF1 promoter activity and transcription by facilitating PRC2 binding and H3K27 trimethylation.
  • Spliced LEF1 NAT antagonizes the action of the unspliced form by competing for promoter interaction.

Conclusions:

  • LEF1 gene expression is attenuated by an antisense non-coding RNA (LEF1 NAT).
  • The regulatory function of LEF1 NAT is controlled by the balance between its spliced and unspliced forms.
  • Alternative splicing of LEF1 NAT plays a critical role in modulating LEF1 expression during epithelial-mesenchymal transition.