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Updated: Apr 12, 2026

Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
The Escherichia coli Envelope Stress Sensor CpxA Responds to Changes in Lipid Bilayer Properties
Rebecca Keller1, Candan Ariöz2, Nicole Hansmeier3
1†Department of Pharmacy and Biochemistry, Johannes Gutenberg-University, D-55128 Mainz, Germany.
Abstract:
The Cpx stress response system is induced by various environmental and cellular stimuli. It is also activated in Escherichia coli strains lacking the major phospholipid, phosphatidylethanolamine (PE). However, it is not known whether CpxA directly senses changes in the lipid bilayer or the presence of misfolded proteins due to the lack of PE in their membranes. To address this question, we used an in vitro reconstitution system and vesicles with different lipid compositions to track modulations in the activity of CpxA in different lipid bilayers. Moreover, the Cpx response was validated in vivo by monitoring expression of a PcpxP-gfp reporter in lipid-engineered strains of E. coli. Our combined data indicate that CpxA responds specifically to different lipid compositions.
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