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Updated: Apr 12, 2026

CD Spectroscopy to Study DNA-Protein Interactions
Published on: February 10, 2022
Spectroscopic Evaluation of DNA-Borate Interactions
Ayse Ozdemir1,2, Omer Faruk Sarioglu1,2, Turgay Tekinay3,4
1Institute of Materials Science & Nanotechnology, Bilkent University, 06800, Bilkent, Ankara, Turkey.
Abstract:
We describe the binding characteristics of two natural borates (colemanite and ulexite) to calf thymus DNA by UV-vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, and a competitive DNA binding assay. Our results suggest that colemanite and ulexite interact with calf thymus DNA under a non-intercalative mode of binding and do not alter the secondary structure of the DNA helix. The FT-IR spectroscopy results indicate that the two borates might interact with DNA through sugar-phosphate backbone binding.
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