[Determination of aniline in whole blood by using solid-phase extraction and high performance liquid chromatography...

To establish a method for the determination of trace aniline in whole blood by using solid-phase extraction and high performance liquid chromatography with fluorescence detector.

After the whole blood was diluted by water, extracted by acetonitrile, cleaned and enriched by Cleanert NH2 solid-phase extraction (SPE) cartridge, separation was performed on a Kromasil C8 column(250 mm x 4. 6 mm i. d., 5 λm) with the mobile phase of water/acetonitrile (20/80, V/V). Detection was carried out by fluorescence detector at ex 225 nm and λem 335 nm.

Calibration curve was linear in the range of 3. 0 - 200.0 µg/L with a correlation coefficient of 0. 9992, and the limit of quantitation(LOQ) was 3. 0 µg/L. The extraction recoveries were 87. 5% - 104. 4%, and the intra-day and inter-day RSDs were 3. 1% - 6. 6% and 6. 4% - 8. 6%, respectively.

The developed method is simple, fast, little interference, good specificity for the satisfactory determination of trace aniline in whole blood.