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Long non-coding RNA MEG3 suppresses migration and invasion of thyroid carcinoma by targeting of Rac1

C Wang, G Yan, Y Zhang

    Neoplasma
    |May 23, 2015
    PubMed

    Insights

    Long noncoding RNA MEG3 is downregulated in papillary thyroid carcinoma (PTC) with metastasis. Overexpressing MEG3 inhibits cancer cell invasion and migration by targeting Rac1, suggesting MEG3 as a potential therapeutic target.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Oncology

    Background:

    • Thyroid cancer is the most common endocrine malignancy.
    • Long noncoding RNAs (Lnc-RNAs) play roles in cancer progression, but their function in thyroid cancer metastasis is unclear.
    • Limited data exists on Lnc-RNA involvement in thyroid cancer invasion and metastasis.

    Purpose of the Study:

    • To investigate the role of Lnc-RNAs, specifically MEG3, in thyroid cancer invasion and metastasis.
    • To explore the molecular mechanism of MEG3's function in papillary thyroid carcinoma (PTC).

    Main Methods:

    • Analysis of MEG3 expression in PTC tissues with and without lymph-node metastasis.
    • Overexpression of MEG3 in PTC cell lines (TPC-1 and HTH83).
    • Dual luciferase reporter assay to confirm the interaction between MEG3 and Rac1.
    • Correlation analysis of MEG3 and Rac1 expression in PTC tissues.

    Main Results:

    • MEG3 was significantly downregulated in PTC tissues with lymph-node metastasis.
    • Downregulated MEG3 expression correlated with lymph-node metastasis.
    • MEG3 overexpression inhibited cell migration and invasion in PTC cell lines.
    • MEG3 negatively regulated Rac1 at the posttranscriptional level.
    • Rac1 expression was inversely correlated with MEG3 expression in PTC tissues.

    Conclusions:

    • MEG3 functions as a tumor suppressor in thyroid cancer.
    • MEG3 inhibits migration and invasion by targeting the Rac1 gene.
    • MEG3 represents a potential therapeutic target for managing thyroid cancer metastasis.
    Keywords:
    Long noncoding RNAMEG3RAC1Thyroid cancermetastasis.

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