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Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection
Published on: November 7, 2018
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Sequence-based genotyping of hepatitis B virus in general population
Ali Karimi1, Ma'soumeh Moezzi2, Reza Imani3
1Associate Professor, Medicinal Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. alikarimi72@skums.ac.ir.
Medical Journal of the Islamic Republic of Iran
|May 23, 2015
Summary
Hepatitis B Virus (HBV) genotype D is likely predominant in Shahrekord, Iran. This study identified genotype D in all detected HBV DNA samples from the general population.
Area of Science:
- Hepatology
- Virology
- Genetics
Background:
- Hepatitis B Virus (HBV) infection is a global health concern, causing acute and chronic liver disease.
- HBV exhibits eight genotypes (A-H) with distinct geographical distributions and varying clinical implications.
- Limited data exists on HBV genotyping within the general population of Southwestern Iran.
Purpose of the Study:
- To determine the predominant Hepatitis B Virus genotypes in the general population of Shahrekord, Southwestern Iran.
- To contribute to the understanding of HBV genotype distribution in underrepresented regions.
Main Methods:
- A cluster sampling method enrolled 3000 individuals from the general population.
- Hepatitis B surface antigen (HBsAg) screening was performed using ELISA.
- HBV DNA extraction, nested PCR detection, and sequencing for genotyping were conducted on HBsAg-positive samples.
Main Results:
- Out of 3000 samples, 40 (1.3%) tested positive for HBsAg.
- HBV DNA was successfully detected in 10 (25%) of the HBsAg-positive samples.
- Hepatitis B Virus Genotype D was identified as the sole genotype among the 10 positive samples.
Conclusions:
- Genotype D is likely the predominant Hepatitis B Virus genotype in the Shahrekord region of Iran.
- Further research is warranted to confirm these findings in a broader population.
