Bioactivity of a Calcium Silicate-based Endodontic Cement (BioRoot RCS): Interactions with Human Periodontal Ligament...

Area of Science:

• Endodontics
• Biomaterials Science
• Cell Biology

Background:

• Tricalcium silicate cements are bioactive, promoting hard tissue formation in dental pulp and bone.
• Root canal filling materials interact with periapical tissues, influencing healing.
• Evaluating new materials like BioRoot RCS against standard sealers (PCS) is crucial for endodontic treatment.

Purpose of the Study:

• To compare the bioactivity and cellular effects of a new tricalcium silicate cement (BioRoot RCS) with a standard zinc oxide-eugenol sealer (PCS).
• To assess the interaction of these root canal sealers with human periodontal ligament (PDL) cells.

Main Methods:

• Human PDL cells were exposed directly to BioRoot RCS and PCS to assess cell viability.
• An in vitro tooth model was used, with root canals filled and apices immersed in culture medium.
• Conditioned media effects on PDL cell proliferation (MTT assay) and growth factor secretion (ELISA) were quantified.

Main Results:

• BioRoot RCS demonstrated significantly less toxic effects on PDL cells compared to PCS.
• BioRoot RCS induced a higher secretion of angiogenic and osteogenic growth factors than PCS.
• These findings indicate enhanced cellular response to the tricalcium silicate cement.

Conclusions:

• The calcium silicate cement (BioRoot RCS) exhibits superior bioactivity compared to the zinc oxide-eugenol sealer (PCS) when interacting with human PDL cells.
• These preclinical findings suggest BioRoot RCS as a promising alternative for root canal sealing.
• Further clinical studies are warranted to confirm these enhanced bioactivity outcomes.