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Updated: Jul 17, 2026
Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Anti-inflammatory Potential of a New Calcium Silicate Based Endodontic Sealer
Thomas Giraud1, Charlotte Jeanneau2, Imad About2
1Aix Marseille Université, Centre National de la Recherche Scientifique, Institut des Sciences du Mouvement, UMR 7287, Marseille, France; Hôpitaux de Marseille, Hôpital Timone, Pôle PROMOD Odonto, Service Réhabilitation Orale et Chirurgicale, Marseille, France.
Introduction:
Persistent inflammation is a critical factor influencing the outcome of endodontic treatment, particularly following removal of necrotic/infected tissues, root filling including a sealer application and a hermetic coronal seal. This study investigates the effect of BioRoot Flow (BRF), Pulp Canal Sealer, and AH Plus endodontic sealers on modulating inflammatory response of human periodontal ligament (hPDL) cells. To this end, we evaluated the effects of the sealers on proinflammatory cytokine secretion by hPDL cells. We also checked the effects on immune cell recruitment in vitro by investigating the sealers' extracts effects on key steps of the inflammatory reaction including monocyte cell adhesion to endothelial cells, their migration using Boyden chambers, and their activation (Figure 1).
Methods:
Extracts were prepared by incubating the sealers in minimum essential medium. To mimic the bacterial infection, hPDL cells were stimulated with lipopolysaccharides and subsequently exposed to the extracts. Proinflammatory interleukin (IL)-6, IL-8, and tumor necrosis factor (TNF)-α cytokine secretion was quantified using enzyme-linked immunosorbent assay. Inflammatory cell recruitment was assessed in vitro using the THP-1 monocytics, focusing on their adhesion to endothelial cells, migration using Boyden chambers and activation by evaluating their adhesion on plastic petri dish surfaces.
Results:
BRF significantly reduced TNF-α and IL-8 secretion levels by 65% and 52%, respectively. By contrast, AH Plus increased IL-8 secretion while Pulp Canal Sealer was associated with increased IL-6 and TNF-α secretion, with 100% and 500% increases, respectively. Furthermore, while all tested sealers decreased the monocyte activation, this decrease was more pronounced with BRF (50%) which was the only one to decrease THP-1 migration (20%).
Conclusions:
This study highlights the endodontic sealers' potential to modulate the inflammatory responses in vitro. BRF mitigated the inflammatory response more than the other materials. It exhibited significant anti-inflammatory properties, suggesting its potential for improving outcomes in endodontic therapy by minimizing the inflammatory response.