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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Related Experiment Video

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Isolation and Culture of Embryonic Mouse Neural Stem Cells
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Is mouse embryonic stem cell technology obsolete?

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  • 1Stem Cell Engineering, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. skarnes@sanger.ac.uk.

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|May 28, 2015
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Summary
This summary is machine-generated.

CRISPR gene editing using Cas9 protein and guide RNAs injected into mouse zygotes enables gene disruption and knock-ins. This advanced CRISPR technology may soon replace traditional embryonic stem cell methods for genetic modification.

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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
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CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Traditional genetic modification often relies on embryonic stem cells.
  • CRISPR-Cas9 technology offers a powerful tool for genome engineering.

Purpose of the Study:

  • To investigate the efficacy of direct CRISPR-Cas9 delivery into mouse zygotes.
  • To assess the potential of this method for gene disruption and knock-ins.

Main Methods:

  • Recombinant Cas9 protein and synthetic guide RNAs were injected into mouse zygotes.
  • CRISPR-Cas9 system was utilized for targeted genetic modifications.

Main Results:

  • Successful gene disruption was achieved in mouse zygotes.
  • Gene knock-ins were facilitated using the CRISPR system.
  • Demonstrated feasibility of direct CRISPR component delivery.

Conclusions:

  • Direct injection of CRISPR-Cas9 components into zygotes is an effective method for genetic modification.
  • This approach shows potential to supersede embryonic stem cell-based techniques in the future.