Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BRD1 haploinsufficiency alters early neuronal programming and disrupts maturation in human induced glutamatergic neurons.

Research square·2026
Same author

Distinct radial glia subtypes regulate midbrain dopaminergic neuron development.

Nature neuroscience·2026
Same author

Prevention of transgene silencing during human pluripotent stem cell differentiation.

Cell stem cell·2026
Same author

BRD1 haploinsufficiency alters early neuronal programming and disrupts maturation in human induced glutamatergic neurons.

bioRxiv : the preprint server for biology·2025
Same author

Development of a biological at-risk volume using apparent diffusion coefficient for parotid-sparing radiation therapy planning.

BJR open·2025
Same author

MJF-14 proximity ligation assay detects early non-inclusion alpha-synuclein pathology with enhanced specificity and sensitivity.

NPJ Parkinson's disease·2024

Related Experiment Video

Updated: Apr 11, 2026

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

17.4K

How to make a midbrain dopaminergic neuron.

Ernest Arenas1, Mark Denham2, J Carlos Villaescusa3

  • 1Laboratory of Molecular Neurobiology, Dept. Medical Biochemistry and Biophysics, Center of Developmental Biology for Regenerative Medicine, Karolinska Institutet, Stockholm 171 77, Sweden ernest.arenas@ki.se.

Development (Cambridge, England)
|May 28, 2015
PubMed
Summary

Understanding midbrain dopaminergic (mDA) neuron development is key for Parkinson's disease (PD) regenerative medicine. Research focuses on in vivo development and in vitro generation of mDA neurons for therapies.

Keywords:
Dopamine neuronsMidbrainParkinson's diseaseRegenerationReprogrammingStem cells

More Related Videos

Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

40.0K
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.7K

Related Experiment Videos

Last Updated: Apr 11, 2026

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells
06:40

Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

Published on: September 15, 2014

17.4K
Primary Culture of Mouse Dopaminergic Neurons
11:58

Primary Culture of Mouse Dopaminergic Neurons

Published on: September 8, 2014

40.0K
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
12:13

Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Published on: July 11, 2019

7.7K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Midbrain dopaminergic (mDA) neuron development is crucial for understanding neurological disorders like Parkinson's disease (PD).
  • Recent advancements aim to leverage this knowledge for therapeutic strategies.

Purpose of the Study:

  • To review recent developments in the molecular mechanisms of mDA neuron development in vivo.
  • To discuss the generation of human mDA neurons in vitro from pluripotent or somatic cells.
  • To identify challenges and future directions for PD regenerative medicine.

Main Methods:

  • Focus on molecular mechanisms regulating mDA neuron development in vivo.
  • Analysis of methods for generating mDA neurons in vitro from stem cells and via direct reprogramming.
  • Review of current literature and research findings.

Main Results:

  • Detailed understanding of molecular pathways governing mDA neuron specification, differentiation, and maintenance.
  • Successful generation of human mDA neurons in vitro using various stem cell-based and reprogramming techniques.
  • Identification of key challenges in translating these findings to clinical applications.

Conclusions:

  • Knowledge of mDA neuron development is vital for advancing PD therapies.
  • In vitro generation of mDA neurons holds promise for cell replacement, disease modeling, and drug discovery.
  • Further research is needed to overcome challenges in regenerative medicine for PD.