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[Effects of chloroquine in reversing multidrug resistance in HNE1/DDP cell line]
Haoxuan Zhang1, Xiaojin Sun, Yiming Sun
1Department of Basic Medical Sciences, Bengbu Medical College, Bengbu 233030, China.E-mail: zhxww@foxmail.com.
Objective:
To investigate the effect of chloroquine in reversing multidrug resistance (MDR) of HNE1/DDP cell line and explore the mechanism.
Methods:
MTT assay was used to detect the cell viability of HNE1 and HNE1/DDP after exposure to different concentrations of DDP (2, 4, 8, 16 and 32 µmol/L) and different concentrations of chloroquine (5, 10, 20, 40, and 80 µmol/L). q-PCR was used to assess the expression of MDR1 mRNA and Western blotting was employed to detect P-glycoprotein (P-gp) expression in HNE1 and HNE1/DDP cells exposed to 5 and 10 µmol/L chloroquine. The cell apoptosis rate of HNE1 and HNE1/DDP cells exposed to 10 and 20 µmol
Results:
Chloroquine exposure caused dose-dependent suppression of the proliferation in both HNE1 and HNE1
Conclusion:
Chloroquine can reverse multidrug resistance in HNE1
