Related Experiment Video
Updated: Apr 11, 2026

Whole-Genome Deoxyribonucleic Acid Extraction from Mycobacterium Species via the Cetyltrimethylammonium Bromide Technique
Published on: December 12, 2025
Direct DNA Extraction from Mycobacterium tuberculosis Frozen Stocks as a Reculture-Independent Approach to
K Bjorn-Mortensen1, J Zallet2, T Lillebaek3
1Department of Epidemiology Research, Statens Serum Institut, Copenhagen, Denmark International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark Greenland's Center of Health Research, Nuuk, Greenland kabm@ssi.dk.
Abstract:
Culturing before DNA extraction represents a major time-consuming step in whole-genome sequencing of slow-growing bacteria, such as Mycobacterium tuberculosis. We report a workflow to extract DNA from frozen isolates without reculturing. Prepared libraries and sequence data were comparable with results from recultured aliquots of the same stocks.

