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PCR with sequence-specific primers for typing of diallelic blood groups
Gabriele Rink1, Erwin A Scharberg, Peter Bugert
1Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, German Red Cross Blood Service Baden-Württemberg - Hessen gGmbH, Heidelberg University, Friedrich-Ebert-Straße 107, 68167, Mannheim, Germany, gabi.rink@medma.uni-heidelberg.de.
Abstract:
PCR with sequence-specific primers (PCR-SSP) is a cost-effective and robust method for the analysis of single nucleotide polymorphisms (SNPs). Many blood group antigens and the antithetic antigens are based on a diallelic SNP in the coding region of the corresponding blood group gene. Here, we describe PCR-SSP protocols for genotyping 24 blood group antigens based on 12 diallelic SNPs. We also provide protocols for molecular determination of the rare blood group phenotypes Yk(a-) and Vel-.
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