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MicroRNA expression profile of surgical removed mandibular bone tissues from patients with mandibular prognathism
Yulou Tian1, Jie Liu2, Xiaofeng Bai3
1Department of Orthodontics, School of Stomatology, China Medical University, Shenyang, People's Republic of China; Department of Orthodontics, Liaoning Institute of Dental Research, Shenyang, People's Republic of China.
Background:
Mandibular prognathism (MP) or skeletal class III malocclusion with a prognathic mandible is one of the most severe facial deformities. Recent work has revealed certain circulating microRNAs (miRNAs) are associated with MP, we conducted this study to characterize the miRNAs expression profile in surgically removed mandibular bone tissue in patients with MP and explored the role of miRNA regulation in the pathogenesis of MP.
Methods:
Affymetrix GeneChip miRNA 3.0 Array was used to examine the miRNA expression in mandibular bone tissues from MP patients and control subjects. A variety of bioinformatic approaches were used to predict the target genes of the miRNAs, find the potential functions and pathways of the target genes, analyze their intersection with differentially expressed mRNAs, and establish miRNA-gene network.
Results:
Eleven upregulated and 11 downregulated miRNAs with a fold change ≥ 2 and a P value <0.05 were identified in bone specimens of MP patients. A total of 3569 genes were predicted as targets of hsa-miR-10a-5p, hsa-miR-150-5p, hsa-miR-192-5p, hsa-miR-194-5p, hsa-miR-197-3p, hsa-miR-30 d-5p, hsa-miR-342-5p and hsa-miR-629-5p, hsa-miR-1202, and hsa-miR-638. The target genes were predicted to be involved in biological functions and signaling pathways related to osteogenesis. Hsa-miR-30 d-5p was the key node of miRNA-gene network.
Conclusions:
Our results indicated a possible association between the differentially expressed miRNAs and MP pathogenesis, and the precise mechanisms are needed to be further validated.
Insights
This study identified specific microRNAs (miRNAs) differentially expressed in mandibular bone tissue of patients with mandibular prognathism (MP). These findings suggest miRNAs play a role in MP pathogenesis, requiring further validation.
Area of Science:
- Oral and Maxillofacial Surgery
- Molecular Biology
- Genetics
Background:
- Mandibular prognathism (MP), a severe facial deformity, involves a prognathic mandible.
- Recent research suggests circulating microRNAs (miRNAs) are linked to MP.
- This study investigates miRNA expression in mandibular bone tissue from MP patients.
Purpose of the Study:
- To characterize the miRNA expression profile in surgically removed mandibular bone tissue of MP patients.
- To explore the role of miRNA regulation in the pathogenesis of MP.
- To identify potential miRNA-gene interactions in MP.
Main Methods:
- Utilized Affymetrix GeneChip miRNA 3.0 Array to analyze miRNA expression in MP and control bone tissues.
- Employed bioinformatic approaches to predict miRNA target genes and their functions.
- Constructed a miRNA-gene network to identify key regulatory interactions.
Main Results:
- Identified 11 upregulated and 11 downregulated miRNAs in MP bone specimens (fold change ≥ 2, P < 0.05).
- Predicted 3569 target genes for several key miRNAs, including hsa-miR-30 d-5p.
- Target genes are involved in osteogenesis-related biological functions and signaling pathways, with hsa-miR-30 d-5p as a central network node.
Conclusions:
- Differentially expressed miRNAs are potentially associated with mandibular prognathism pathogenesis.
- Further research is required to validate the precise molecular mechanisms involved.
- Identified key miRNAs and their target genes offer insights into MP development.
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