[Bone marrow mesenchymal stem cells suppress E coli-induced bacterial prostatitis in rats]

Area of Science:

• Regenerative Medicine
• Urology
• Immunology

Background:

• Prostatitis, particularly bacterial prostatitis, remains a challenging condition with limited effective treatments.
• Inflammation plays a central role in the pathogenesis of prostatitis, involving cytokines like IL-1β and TNF-α.
• Bone marrow mesenchymal stem cells (BMSCs) exhibit immunomodulatory and regenerative properties with potential therapeutic applications.

Purpose of the Study:

• To evaluate the efficacy of BMSCs in treating E. coli-induced acute bacterial prostatitis (ABP) and chronic bacterial prostatitis (CBP) in a rat model.
• To investigate the impact of BMSC transplantation on prostatic histopathology and inflammatory markers.

Main Methods:

• BMSCs were isolated and cultured. Rats were induced with E. coli to develop ABP or CBP and treated with BMSCs or PBS.
• Prostate tissues were analyzed for histopathological changes, and mRNA and protein levels of IL-1β and TNF-α were quantified using RT-PCR and ELISA.

Main Results:

• BMSC treatment significantly ameliorated histopathological changes in ABP and CBP models, including reduced edema and fibrous hyperplasia.
• Levels of IL-1β and TNF-α mRNA and protein were significantly decreased in BMSC-treated groups compared to untreated prostatitis groups.
• These inflammatory markers in BMSC-treated groups were closer to levels observed in normal control rats.

Conclusions:

• BMSCs demonstrate a significant inhibitory effect on E. coli-induced prostatitis in rats.
• The therapeutic effect of BMSCs may be attributed to the reduction of prostatic inflammation, inflammatory cell infiltration, and the expression of IL-1β and TNF-α.